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Heteronuclear correlation experiments obey the same principles as homonuclear correlation experiments but require more complex execution The problem with any type of heteronuclear NMR experiment is that heteronuclei have different nuclear g-factors, gI , and as a result have substantially different sensitivities given wide potential variations in spin state energy level populations This situation arises because values of gI are directly proportional to the Larmor precession frequency, L (according to Equation (59)), and as values of L decline so too does the spin state population imbalance between spin states (according to the Boltzmann Equation (513)) In other words, the smaller is gI , the less is the population difference between spin states and hence the less sensitive is the nucleus concerned.

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(gpwis not so simple in the curved spacetime of general relativity; there the metric tensor is not fixed in advance, but is determined by the Einstein equations). For flat spacetime the covariant and contravariant metric tensors are identical, gpw = gPW, (1.22) and their contraction on one index yields the four-dimensional Kronecker delta

Nevertheless, spin spin coupling relationships are possible between any number of spin-active nuclei such as 1 H, 13 C, 15 N, 31 P or 19 F, making heteronuclear correlation experiments potentially very valuable The pulse sequence of a 2D heteronuclear single quantum coherence (HSQC) correlation experiment is shown to illustrate the additional complexity required in the pulse sequence relative to the pulse sequences of the homonuclear correlation experiments (Figure 512) This HSQC correlation experiment requires that there is a preparation period during which nuclei with the highest nuclear g-factor (or ratio) are subjected to radio-frequency pulses in order to effect polarisation transfer to spin-coupled source nuclei I, with the lower nuclear g-factor (or ratio) Thereafter, in time t1 , transverse magnetisation is generated involving nuclei I that evolves with chemical shift.

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<table border="1"> <tr><td>Longer Text 1</td><td>Longer Text 2</td></tr> </table> </p> <p> Mixed Text Table<br /> <table border="1"> <tr><td>Short Text</td><td>Even Longer Text</td></tr> </table> </p> </body> </html> FIGURE 9-2

Additional pulse sequences during time m then ensure reverse magnetisation transfer from I to spin-coupled destination heteronuclei S, and afterwards nal coherence is observed involving nuclei S and acquired during t2 The HSQC experiment is routinely used to identify heteronuclear 1] spin spin coupled nuclei such as 1 H 13 C or 1 H 15 N pairs, and is the basis of many so-called double-resonance experiments used for the structural determination of proteins and of other biological macromolecules, as we shall see later A variation on the HSQC experiment is the heteronuclear multiple bond correlation (HMBC) experiment This is a sensitive technique that may be used to identify heteronuclear 2 ] and 3] spin spin coupled nuclei..

8. We have k 0.01. The boundary condition on the left is that the normal derivative is zero. Now if we were at an inner point, we would have, by (5), u0, j

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